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osm  (R&D Systems)


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    R&D Systems osm
    Osm, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oncostatin+m/Recombinant+Human+Oncostatin+M+(OSM)+Protein/pm41986711-647-160-164
    Average 95 stars, based on 144 article reviews
    osm - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Exploring the potential of liver microphysiological systems of varied configurations to model cholestatic chemical effects.
    Article Snippet: PHH maintenance medium was used for cell culture from day 1 until the end of each experiment; it consisted of William’s E medium with PHH maintenance supplements (4% cocktail B, 1 nM dexamethasone; CM4000, ThermoFisher). .. Human iHeps (iCell Hepatocytes 2.0, iHeps 2.0) were purchased from FujiFilm-Cellular Dynamics International (C1023, lot# 103934; Santa Ana, CA). iCell Hep plating medium consisted of RPM 1640 phenol red-free media (11835030, ThermoFisher) with 2% B-27 supplement (17504044, ThermoFisher), 100 nM dexamethasone (D9184, Millipore Sigma), 25 μg/mL gentamicin (1574044, ThermoFisher), and 20 ng/mL oncostatin M(295-OM-010, R&D Systems, Minneapolis, MN); it was used for pre-differentiation of iHeps according to the manufacturer’s protocol. iHep maintenance medium consisted of RPMI 1640 phenol- red free media, 2% B-27, 100 nM dexamethasone, and 25 μg/mL gentamicin; it was used for cell culture in the OrganoPlate 2-lane 96 and OrganoPlate 3-lane 40 devices and in the 96-well plates. .. 1 3 thawed into CHRM (CM7000, ThermoFisher) and HepaRG were thawed into Thawing and Plating medium, centrifuged at 100 × g for 10 min, resuspended in plating medium (specific for each cell type as detailed above) and then seeded into the PhysioMimix LC12 scaffold at a seeding density of ~ 600,000 cells/chip as detailed elsewhere (Lim et al. 2023).

    Article Title: Ether lipids influence cancer cell fate by modulating iron uptake
    Article Snippet: .. MCF7 (ATCC, HTB-22) cells were cultured in Dulbecco’s Modified Eagle Medium GlutaMAX (DMEM, ThermoFisher Scientific, 61965059) supplemented with 10% Fetal Bovine Serum and Penicillin-Streptomycin mixture (BioWhittaker/Lonza, DE17-602E) and treated with oncostatin M (OSM, R&D systems, 295-OM-050, 100 ng/mL, 72 h). ..

    Article Title: Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
    Article Snippet: .. After RA treatment, organoids embedded in Matrigel drop are cultured in Hepatocyte culture medium (HCM Lonza, Walkersville, MD) with 10 ng/mL hepatocyte growth factor (HGF; PeproTech, Rocky Hill, NJ), 0.1 μM Dexamethasone (Dex; Sigma) and 20 ng/mL Oncostatin M (OSM; R&D Systems). ..

    Article Title: Ether lipids influence cancer cell fate by modulating iron uptake.
    Article Snippet: .. MCF7 (ATCC, HTB-22) cells were cultured in Dulbecco’s Modified Eagle Medium GlutaMAX (DMEM, ThermoFisher Scientific, 61965059) supplemented with 10% Fetal Bovine Serum and Penicillin-Streptomycin mixture (BioWhittaker/Lonza, DE17-602E) and treated with oncostatin M (OSM, R&D systems, 295-OM-050, 100 ng/mL, 72 h). ..

    other:

    Article Title: Hepato-biliary-pancreatic tissues and methods of making same
    Article Snippet: By “inducing factors,” it is meant a factor used to direct differentiation, for example, retinoic acid (RA; Sigma, MO, USA), hepatocyte growth factor (HGF; PeproTech, NJ, USA), 0.1 μM Dexamethasone (Dex; Sigma) and 20 ng/mL Oncostatin M (OSM; R&D Systems) for hepatocyte differentiation.

    Modification:

    Article Title: Ether lipids influence cancer cell fate by modulating iron uptake
    Article Snippet: .. MCF7 (ATCC, HTB-22) cells were cultured in Dulbecco’s Modified Eagle Medium GlutaMAX (DMEM, ThermoFisher Scientific, 61965059) supplemented with 10% Fetal Bovine Serum and Penicillin-Streptomycin mixture (BioWhittaker/Lonza, DE17-602E) and treated with oncostatin M (OSM, R&D systems, 295-OM-050, 100 ng/mL, 72 h). ..

    Article Title: Ether lipids influence cancer cell fate by modulating iron uptake.
    Article Snippet: .. MCF7 (ATCC, HTB-22) cells were cultured in Dulbecco’s Modified Eagle Medium GlutaMAX (DMEM, ThermoFisher Scientific, 61965059) supplemented with 10% Fetal Bovine Serum and Penicillin-Streptomycin mixture (BioWhittaker/Lonza, DE17-602E) and treated with oncostatin M (OSM, R&D systems, 295-OM-050, 100 ng/mL, 72 h). ..



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    Image Search Results


    Topical calcitriol application ameliorates IMQ‐induced psoriasis in mice. (A) Schematic illustration of the unilateral ear model experimental design. (B) Serum calcium levels. (C) Body weight changes. (D) Clinical severity scores (erythema, scaling and thickness) of the right ear, assessed on indicated days using a 0–4 scale. Cumulative scores are presented as mean ± SD. (E) Representative photographs of the right ear at endpoint. (F) Haematoxylin and eosin (H&E) staining of right ear sections. Scale bar = 100 μm. (G) Relative mRNA expression levels of IL‐1α, IL‐6, IL‐17A and IL‐23A . Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Downregulating CHI3L2 via the STAT3 Pathway: The Mechanism of Calcitriol in Suppressing Psoriatic Inflammation and Keratinocyte Hyperproliferation

    doi: 10.1111/jcmm.71367

    Figure Lengend Snippet: Topical calcitriol application ameliorates IMQ‐induced psoriasis in mice. (A) Schematic illustration of the unilateral ear model experimental design. (B) Serum calcium levels. (C) Body weight changes. (D) Clinical severity scores (erythema, scaling and thickness) of the right ear, assessed on indicated days using a 0–4 scale. Cumulative scores are presented as mean ± SD. (E) Representative photographs of the right ear at endpoint. (F) Haematoxylin and eosin (H&E) staining of right ear sections. Scale bar = 100 μm. (G) Relative mRNA expression levels of IL‐1α, IL‐6, IL‐17A and IL‐23A . Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: To mimic psoriatic keratinocyte dysfunction, HaCaT cells were incubated with 10 ng/mL M5 (IL‐17A, IL‐22, IL‐1α, oncostatin M and TNF‐α) (ABclonal Technology Co. Ltd., Wuhan, China) for 24 h as reported previously [ , , , ].

    Techniques: Staining, Expressing

    Topical calcitriol treatment ameliorates distant psoriatic skin lesions in the bilateral ear model. (A) Schematic illustration of the bilateral ear model experimental design. (B) Serum calcium levels. (C) Body weight changes. (D) Clinical severity scores (erythema, scaling and thickness) of the right ear, assessed on indicated days using a 0–4 scale. Cumulative scores are presented as mean ± SD. (E) Representative photographs of the left ear at endpoint. (F) Haematoxylin and eosin (H&E) staining of left ear sections. Scale bar = 100 μm. (G) Relative mRNA expression levels of IL‐1α, IL‐6, IL‐17A and IL‐23A . Data are presented as mean ± SD. ** p < 0.01, **** p < 0.0001.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Downregulating CHI3L2 via the STAT3 Pathway: The Mechanism of Calcitriol in Suppressing Psoriatic Inflammation and Keratinocyte Hyperproliferation

    doi: 10.1111/jcmm.71367

    Figure Lengend Snippet: Topical calcitriol treatment ameliorates distant psoriatic skin lesions in the bilateral ear model. (A) Schematic illustration of the bilateral ear model experimental design. (B) Serum calcium levels. (C) Body weight changes. (D) Clinical severity scores (erythema, scaling and thickness) of the right ear, assessed on indicated days using a 0–4 scale. Cumulative scores are presented as mean ± SD. (E) Representative photographs of the left ear at endpoint. (F) Haematoxylin and eosin (H&E) staining of left ear sections. Scale bar = 100 μm. (G) Relative mRNA expression levels of IL‐1α, IL‐6, IL‐17A and IL‐23A . Data are presented as mean ± SD. ** p < 0.01, **** p < 0.0001.

    Article Snippet: To mimic psoriatic keratinocyte dysfunction, HaCaT cells were incubated with 10 ng/mL M5 (IL‐17A, IL‐22, IL‐1α, oncostatin M and TNF‐α) (ABclonal Technology Co. Ltd., Wuhan, China) for 24 h as reported previously [ , , , ].

    Techniques: Staining, Expressing

    Calcitriol ameliorates M5‐induced abnormal proliferation, migration and inflammatory responses in HaCaT cells. (A) Protein expression levels of KRT1 and KRT6 were determined by Western blot analysis, with β‐Actin serving as the internal loading control. (B) Cell viability was assessed in different treatment groups. (C) Colony formation assay was performed to evaluate the proliferative capacity of cells in each group. (D) Representative images of wound healing assay at 0 and 24 h post‐treatment. Scale bar = 400 μm. (E) Relative mRNA expression levels of IL‐1α, IL‐6, IL‐17A and IL‐23A . Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Downregulating CHI3L2 via the STAT3 Pathway: The Mechanism of Calcitriol in Suppressing Psoriatic Inflammation and Keratinocyte Hyperproliferation

    doi: 10.1111/jcmm.71367

    Figure Lengend Snippet: Calcitriol ameliorates M5‐induced abnormal proliferation, migration and inflammatory responses in HaCaT cells. (A) Protein expression levels of KRT1 and KRT6 were determined by Western blot analysis, with β‐Actin serving as the internal loading control. (B) Cell viability was assessed in different treatment groups. (C) Colony formation assay was performed to evaluate the proliferative capacity of cells in each group. (D) Representative images of wound healing assay at 0 and 24 h post‐treatment. Scale bar = 400 μm. (E) Relative mRNA expression levels of IL‐1α, IL‐6, IL‐17A and IL‐23A . Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: To mimic psoriatic keratinocyte dysfunction, HaCaT cells were incubated with 10 ng/mL M5 (IL‐17A, IL‐22, IL‐1α, oncostatin M and TNF‐α) (ABclonal Technology Co. Ltd., Wuhan, China) for 24 h as reported previously [ , , , ].

    Techniques: Migration, Expressing, Western Blot, Control, Colony Assay, Wound Healing Assay

    Calcitriol ameliorates M5‐induced psoriasis‐like phenotypes through CHI3L2 downregulation. (A) The mRNA expression of RPL36A, PPBP, UBE2F, MGC32805, IL4I1, LINC00519, CHI3L2, ABHD14A and PCDHGB7 , validated by RT‐qPCR assay. (B) Efficacy of shRNA‐mediated CHI3L2 knockdown confirmed by RT‐qPCR. (C) Protein expression levels of KRT1 and KRT6 were determined by Western blot analysis, with β‐Actin serving as the internal loading control. (D) Cell viability was assessed in different treatment groups. (E) Representative images of wound healing assay at 0 and 24 h post‐treatment. Scale bar = 400 μm. (F) Relative mRNA expression levels of IL‐1α, IL‐6, IL‐17A and IL‐23A . Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Downregulating CHI3L2 via the STAT3 Pathway: The Mechanism of Calcitriol in Suppressing Psoriatic Inflammation and Keratinocyte Hyperproliferation

    doi: 10.1111/jcmm.71367

    Figure Lengend Snippet: Calcitriol ameliorates M5‐induced psoriasis‐like phenotypes through CHI3L2 downregulation. (A) The mRNA expression of RPL36A, PPBP, UBE2F, MGC32805, IL4I1, LINC00519, CHI3L2, ABHD14A and PCDHGB7 , validated by RT‐qPCR assay. (B) Efficacy of shRNA‐mediated CHI3L2 knockdown confirmed by RT‐qPCR. (C) Protein expression levels of KRT1 and KRT6 were determined by Western blot analysis, with β‐Actin serving as the internal loading control. (D) Cell viability was assessed in different treatment groups. (E) Representative images of wound healing assay at 0 and 24 h post‐treatment. Scale bar = 400 μm. (F) Relative mRNA expression levels of IL‐1α, IL‐6, IL‐17A and IL‐23A . Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: To mimic psoriatic keratinocyte dysfunction, HaCaT cells were incubated with 10 ng/mL M5 (IL‐17A, IL‐22, IL‐1α, oncostatin M and TNF‐α) (ABclonal Technology Co. Ltd., Wuhan, China) for 24 h as reported previously [ , , , ].

    Techniques: Expressing, Quantitative RT-PCR, shRNA, Knockdown, Western Blot, Control, Wound Healing Assay

    ( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and OSM signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with recombinant OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.

    Journal: bioRxiv

    Article Title: Dysregulated tissue-resident lymphocytes drive senile emphysema by impairing alveolar regeneration

    doi: 10.64898/2026.04.22.720146

    Figure Lengend Snippet: ( A ) CellChat analysis of TRL subset interactions with AT2s. ( B ) Heatmap showing the interactions of IFNγ and OSM signaling pathways from TRL subsets to AT2s, analyzed by CellChat. ( C ) Plots showing the expression of Osm and Ifng in young and aged TRL subsets. ( D ) Images of organoids derived from AT2s of young mice co-cultured with TRL subsets from young or aged mice. ( E and F ) Quantification of colony-forming efficiency (CFE) and organoid size. ( G ) Images of organoids derived from AT2s of young mice treated with recombinant OSM or IFNγ. ( H and I ) Quantification of colony-forming efficiency (CFE) and organoid size.

    Article Snippet: Where applicable, 1 ng/mL recombinant mouse IFNγ (Cat#BE0055-1MG; BioXcell) and 20 ng/mL recombinant mouse OSM (Cat#50112-M08H; SinoBiological) were added to the medium.

    Techniques: Protein-Protein interactions, Expressing, Derivative Assay, Cell Culture, Recombinant